Staining process has begun, no stat slides can be added in the batch. The thickness of the spread when pulling the smear is determined by the 1angle of the spreader slide the greater the angle, the thicker and shorter the smear. Blood smear preparation requires two clean glass slides frosted at one end. In this way a thin smear with uninjured host cells and possible protozoans and or microfilariae will be obtained. The purpose of preparation of blood smear blood film is to study the morphology of rbcs, differential leukocyte count and reticulocyte count. Blood smears are useful for examining cell morphology and identifying bloodborne parasites. Remember to include a detailed history and description of lesions. Blood smear prepared for you by an experienced technician smear evaluation performed by a technician on every specimen. Label precleaned slides preferably frostedend with patients name or other identifier, date and time of collection. Making and staining a blood smear a wellmade blood smear is a beauty to behold, and likely to yield interesting and significant information for a research project.
Always mix the specimen immediately before making the smear by gently rolling the tube several times to ensure good cellular distribution, dont shake or invert. Note that the diameter of the red blood cell is similar to the diameter of the small lymphocyte nucleus. Attention to detail in slide preparation, staining and standard criteria for cell estimates, morphology and differential reporting enhance the value of results provided to the physician. There is mild variation in size anisocytosis but no real variation in shape poikilocytosis. Prior to staining, fix the material to be observed over the slide. The smear is stained with wrights stain and performed after the complete blood count is run. Troubleshooting blood smear errors problem solutions short smear use a larger droplet of blood.
Many hematological diagnoses depend upon this procedure and often a definitive diagnosis can be established from the careful examination of the blood film. Request pdf guidelines for blood smear preparation and staining procedure. Clean, highquality glass microscope slides are essential to blood smear preparation. Smears made from edtaanticoagulated blood should be made. Preparation of blood smears in order to reduce the number of unacceptable smears received, marshfield labs recommends the following procedure to be used in preparation of blood smears. View information on preparing a blood smear slide for ideal rapid diagnosis by fish.
The thickness of smear layer found with the use of different endodontic instrument in increasing order was found to be as hand files, profiles and protapers 4. It takes about 510 minutes to stain a batch of smears. Blood smears for rapid diagnosis by fish abbott molecular. The extra time and care taken during the field season will be rewarded later when the smears must be scanned, and parasites identified and counted.
Place small drop of blood near frosted end of smear. Slides are just automatically dipped in the stain in the buffer and a series of rinses. Devices for blood smear preparation fisher scientific. Blood smears are useful for examining cell morphology and identifying blood borne parasites. Proper blood smear preparation texas veterinary medical. How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium. The blood is streaked in a thin film over the slide. The peripheral blood film remains a very useful tool in hematological assessment. This picture illustrates the proper procedure for making blood smears. Peripheral blood smear examination macroscopic view. Three mechanical devices for preparing blood smears were compared to the traditional coverslip and manual wedge methods.
White blood cells wbcs, leukocytes help fight infections or participate in immune responses. Notice the angle and direction that the spreader slide is pulled, first back towards the drop of blood, then quickly forward to the end of the slide. Clean slides with 70 to 90% alcohol and allow to dry. Place a small drop of blood, or one side about 12 cm from one end.
The blood droplet on the right is the preferred size. Jan 09, 2017 a blood smear is often used as a followup test to abnormal results on a total blood count cbc to evaluate the various types of blood cells. A series of five slides was prepared from each sample of blood. The blood smear allows for the evaluation of these cells. A well prepared blood smear is necessary for microscopic examination of blood. Preparation of thick and thin blood films on the same slide. Alternately, edta anticoagulated blood may be used if the smear is made within one. Slides should not be left uncovered on top of the counter. Aqueous solutions of stains are stable only after 36. Examination of the peripheral blood smear is an inexpensive but powerful diagnostic tool in both children and adults. Why is it important to limit the quantity of cells used to prepare a smear. A blood smear is a blood test used to look for abnormalities in blood cells. Set the second slide in front of blood drop at 30 to 45degree angle. Apr 23, 2014 just recently i emailed my friend and said funny well, not really how many psychologists, doctors, dieticians, support groups, hospitals, and even a hypnotist, i went to over the years that couldnt help me and all it took in the end was a simple site like this, and a supportive partner.
Mar 29, 2016 initially, the blood smear test was done manually using a microscope. Peripheral blood or potassium edta anticoagulated blood 12 mg edta1 ml blood may be used. Blood smear preparation properly prepared blood smears are essential to accurately assess the patients status. This method produces a gradual decrease in thickness of the blood from thick to thin ends with the smear terminating in a feathered edge approximately 2 mm long.
A blood smear is often used as a followup test to abnormal results on a total blood count cbc to evaluate the various types of blood cells. The clinical pathology laboratory uses the wedge technique for preparation of blood smears. Dec, 2016 the peripheral smear, a window into the human body and hematology devapiran jaishankar, m. Dec 08, 2006 a well prepared blood smear is necessary for microscopic examination of blood. Guidelines for blood smear preparation and staining procedure for. The three main blood cells that the test focuses on are. Standardization of blood smears prepared in transparent acetate. This file is licensed under the creative commons attributionshare alike 3. It is important to keep gentle, equal pressure throughout the whole process, and do not lift the top slide before it. Long smear no feathered edge use a smaller drop of blood. A peripheral blood smear pbs or blood film is required to be made from capillary blood or from a drop of blood from an edta anticoagulated blood sample. The peripheral smear, a window into the human body and hematology devapiran jaishankar, m.
Why are all colored dyes not necessarily useful for simple staining. The slide is allowed to airdry and is then stained specimen edta anticoagulated blood is preferred. The other is pusher or the spreader slide drop of blood 23 mm in diameter. Initially, the blood smear test was done manually using a microscope. The blood smear should occupy the central portion of the slide and should not touch the edges. To spread the blood, rapidly but gently push the top slide forward through the remainder of the smear. You may do so in any reasonable manner, but not in. A wellmade blood smear is a beauty to behold, and likely to yield interesting and. What links here related changes upload file special pages permanent link page information. In some ways it is becoming a lost art but it often provides rapid, reliable access to information about a variety of hematologic disorders. Introduction to peripheral blood smear examination clinical. Blood filled capillary tube or purple top with diffsafe dispenser.
For more information on how to make a good blood smear, please also refer to related links below. But, with the advancement of technology, automated digital systems have become available that help to analyze blood smears in a more efficient manner. All authors read and approved the final manuscript. Pdf feline blood smear preparation and evaluation researchgate. Pdf when carrying out a complete blood count, preparation and evaluation of a blood smear should always be performed alongside. Blood smears are needed for microscopic examination of the blood. Introduction to peripheral blood smear examination. The smear and simple stains san diego miramar college. Peripheral blood smear test purpose, procedure, results. This procedure describes the preparation of thin blood films. The size of the drop of blood and acuteness of the angle formed between the slides, will determine the thickness of the film, a more acute angle resulting in a thicker film. To the right is a typical neutrophil with the usual 3 segmentations of the nucleus. Learn preparing staining a blood smear with free interactive flashcards. What problems can arise when the slide is heated in a flame.
The examination of the peripheral blood smear is an important basic hematological procedure. Edta causes red blood cells to enlarge and lyse and will distort parasites making speciation difficult. Do not touch the surface of the slide where the blood smear will be made. Blood smears can be made from the blood remaining in the needle by pushing a small drop of blood onto the slide with the last evacuated tube.
Use of sodium hypochlorite with edta was found to be more effective in reducing or removing the smear layer. Making and staining a blood smear university of vermont. Just recently i emailed my friend and said funny well, not really how many psychologists, doctors, dieticians, support groups, hospitals, and even a hypnotist, i went to over the years that couldnt help me and all it took in the end was a simple site like this, and a supportive partner. Without delay place a spreader at an angle of 45 from the slide and move it back to make contact with the drop. Ideally, blood smear evaluation should be performed on a properly prepared blood smear to improve the assessment of all cell lines. Routine examination of blood films for malaria parasites. Select two glass slides that are clean and free of chipped edges. If you are using venous blood, blood smears should be prepared as soon as possible after collection. Neel, dvm, dacvp clinical associate professor, clinical pathology nc state college of veterinary medicine raleigh, nc, 27607 introduction although tremendous advances have been made in the field of pointofcare hematology analyzers, examination of a well prepared, well stained blood smear remains the cornerstone of veterinary.
Choose from 500 different sets of preparing staining a blood smear flashcards on quizlet. Due to a lack of guidance documents, many organizers are reluctant to offer a. A properly prepared blood smear is essential to accurate assessment of cellular morphology. A blood filmor peripheral blood smearis a thin layer of blood smeared on a glass. Principle of smear preparation smear preparation a small drop of blood is placed near the frosted end of a clean glass slide. Hold top slide securely and pull back into entire drop of blood. Glass slides should be free of chipped edges, glass fragments, dust, powder, fingerprints, and other debris, as these can affect blood spreading. For preparation of a smear on a slide, what is the purpose of heat fixation. May 01, 2015 peripheral blood smear examination macroscopic view.
An extensive study was undertaken to compare five methods of blood smear preparation. A variety of methods are available for preparing and staining blood smears, the most common of which are discussed in this atlas. And no special preparation is needed for this test. Comparison of five methods for preparing blood smears. If preparation is not fixed or sticked over the slide, the cells are washed off during staining. Hccstafford campus 1 laboratory 2 smear preparation, simple stain and bacterial morphology laboratory notes are to be used as a study guide only and do not represent the comprehensive information you will need to know for the exams. A blood smear is a snapshot of the cells that are present in the blood at the time the sample is obtained. Red blood cells display normal orange pink cytoplasm with central pallor 12 the diameter of the red cell. Blood smears are used to determine leukocyte differentials, to evaluate erythrocyte, platelet and leukocyte morphology, and, if necessary, to estimate platelet and leukocyte counts. Practical of clinical hematology islamic university of gaza. This new methylene bluestained blood smear shows large numbers of heavily stained reticulo. Preparation thin blood films and giemsa staining lqm laboratory. This occurs when there is inadequate contact with the stain solutions. The blood can be obtained from finger heal microhematochrit tubes non heparinised for edta or heparinised for capillary blood wooden stick or glass capillary pipette.
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